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ShiyaLiua1ZhiyuanWangb1DaoqiZhua1JiabinYangaDandanLouaRuijiaoGaoaZetaiWangaAiwuLibYingLvbQinFana
a
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China
b
NanFang Hospital, Guangzhou, 510515, China
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A significant difference in the genome-wide methylation status between Nasopharyngeal carcinoma CNE-2 cells and its radioresistant strain.
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Shengmai Yin-mediated enhancement of radiosensitivity might be mediated by restoration of its original DNA methylation status.
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Tenascin-C was downregulated and restored to partially hypermethylated in CNE-2R after Shengmai Yin intervention, DNMT3a maybe the key protein of DNA methylation of TNC.
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The downregulation of TNC inhibited NPC cell radiation resistance, migration and invasion.
Shengmai Yin (SMY) is a Chinese herbal decoction that effectively alleviates the side effects of radiotherapy in various cancers and helps achieve radiotherapy's clinical efficacy. In this study, we explored the interaction mechanism among SMY, DNA methylation, and nasopharyngeal carcinoma (NPC). We identified differences in DNA methylation levels in NPC CNE-2 cells and its radioresistant cells (CNE-2R) using the methylated DNA immunoprecipitation array and found that CNE-2R cells showed genome-wide changes in methylation status towards a state of hypomethylation. SMY may restore its original DNA methylation status, and thus, enhance radiosensitivity. Furthermore, we confirmed that the differential gene Tenascin-C (TNC) was overexpressed in CNE-2R cells and that SMY downregulated TNC expression. This downregulation of TNC inhibited NPC cell radiation resistance, migration, and invasion. Furthermore, we found that TNC was hypomethylated in CNE-2R cells and partially restored to a hypermethylated state after SMY intervention. DNA methyltransferases 3a may be the key protein in DNA methylation of TNC.
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